Stable Cell Line development

At Profacgen, we use lentiviral vector for efficient gene delivery and to achieve high integration rate. Limited dilution and antibiotics screening are then applied to select for high-yield, genetically homogenous stable expression monoclones. When fluorescent protein is fused to the target protein, we can also use flow cytometry to screen for positive clones. Profacgen also offers the generation of Tet-ON inducible stable cell lines for inducible expression of your target gene. Doxycycline can then be used to induce the expression of your target gene.